Summary and Conclusions

Heparin and heparan sulfate are complex glycosaminoglycans that play multiple roles in the normal physiology and pathophysiology of the human body. The structural diversity displayed by these GAG chains makes them extremely challenging molecules to analyze. Also unlike DNA and proteins, it is not possible to amplify these structures, as their biosynthesis is not template driven. Therefore in cases where sample amounts are limited (e.g., isolated cell surface HS), it further magnifies the...

Info

Figure 1 Size-exclusion chromatography elution profile of partial enzymatic digest of porcine mucosal-derived heparan sulphate (PMHS). Separation was carried out on two Amersham XK-16 columns (15 mm x 1 m) connected in series packed with Superdex 30 with a 0-1000 min run time in ammonium bicarbonate 0.5 M at a flow rate of 0.5 ml min. Superdex 30n (11) can be employed and give excellent and consistent resolution. In addition this column can be coupled to the HPLC and run at faster flow rates,...