Info

Brain, mammary gland, prostate, small intestine, testis, uterus *, not reported. **, variant form. identified a HS-polymerase, EXT2, by direct peptide sequencing of that purified from bovine serum (86). The bovine EXT2 has a type II transmembrane protein composed of 718 amino acids. The recombinant bovine EXT2 showed both HS-GlcAT-II and GlcNAcT-II activities toward GlcNAc- GlcA-GlcNAc and GlcA-GlcNAc oligosaccharide acceptors, respectively. EXT1 and EXT2 form a hetero-dimeric complex in vivo,...

Summary and Conclusions

Heparin and heparan sulfate are complex glycosaminoglycans that play multiple roles in the normal physiology and pathophysiology of the human body. The structural diversity displayed by these GAG chains makes them extremely challenging molecules to analyze. Also unlike DNA and proteins, it is not possible to amplify these structures, as their biosynthesis is not template driven. Therefore in cases where sample amounts are limited (e.g., isolated cell surface HS), it further magnifies the...