Immunofluorescence (IF) pattern studies are very helpful in grouping the immunologic disorders. They can also be used to map the location of the dermal-epidermal split in the hereditary bullous disorders. A diagram and description of each method commonly used for skin disorders is shown in Figure 2.
■ Direct immunofluorescence (DIF) (Fig. 2A)
■ Indirect immunofluorescence (IIF) (Fig. 2B)
■ Immunoblotting (IB) to determine molecular weight of antigens
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