Fig. 3. RNA extraction using a phenol-chloroform methodology is possible even with small tissue samples. (A) Coronal section showing staining of mPer2 by in situ hybridization, illustrating region of sampling and high mPer2 expression. (B) Quantitative real-time polymerase chain reaction data from six pooled suprachiasmatic nuclei punches, illustrating clear circadian rhythm in mPer2 expression. RNA was extracted in 0.5 mL monophasic lysis reagent.
3. Wear laboratory gloves at all times, and change them whenever the RNase-free working area is re-entered.
4. Exercise particular care with new lab users to ensure that they are aware of the ubiquitous nature of RNases, and are familiar with the necessary precautions.
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