Amc1127

Fig. 5. Bioluminescence from the dual-reporter stain, AMC1127, was measured using a cooled charge-coupled device camera. AMC1127 carries two promoter fusions in its chromosome: PpsbAI::luxAB in NS1 and PkaiBC::luc in NS2. Cells were patched onto duplicate BG-11M plates. The upper plate was provided with gaseous «-decanal to monitor transcription from the psbAI promoter, whereas the lower plate received 5 mM luciferin to examine the bioluminescence from the kaiBC promoter. Light produced from the luciferase reaction was detected by the camera during a 1-min exposure when the lights were turned off (right). Data were saved and processed by the MetaMorph program. The intensity of light produced over time can be plotted to display the circadian rhythm of each patch of cells in counts per minute.

+ decanal

+ luciferin

Fig. 5. Bioluminescence from the dual-reporter stain, AMC1127, was measured using a cooled charge-coupled device camera. AMC1127 carries two promoter fusions in its chromosome: PpsbAI::luxAB in NS1 and PkaiBC::luc in NS2. Cells were patched onto duplicate BG-11M plates. The upper plate was provided with gaseous «-decanal to monitor transcription from the psbAI promoter, whereas the lower plate received 5 mM luciferin to examine the bioluminescence from the kaiBC promoter. Light produced from the luciferase reaction was detected by the camera during a 1-min exposure when the lights were turned off (right). Data were saved and processed by the MetaMorph program. The intensity of light produced over time can be plotted to display the circadian rhythm of each patch of cells in counts per minute.

calculated for each exposure throughout the experiment. These data can then be exported to Microsoft Excel and analyzed by graphic representation.

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