Northern Blotting

Figure 7.13a shows a blotting technique that can determine the size of an mRNA and tell us about its expression patterns. RNA is denatured by heating to remove any intramolecular double-stranded regions and then electrophoresed on a denaturing agarose gel. The RNA is transferred to a nylon membrane (as described on page 136 for the transfer of DNA). The nylon membrane is incubated with a radiolabeled, single-stranded cDNA probe

Table 7.2. Blotting Techniques

What Is Probed Nature of the Probe Book Page for Description

Southern blot Northern blot Western blot

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