(page 143), or an antisense RNA probe (page 147). Following hybridization, excess probe is washed off and the nylon membrane exposed to X-ray film. The mRNA is visualized on the autoradiogram (page 139) because it hybridized to the radioactive probe. By analogy with Southern blotting, this technique is called northern blotting (Table 7.2). Figure 7.13ft shows a northern blot for a cytochrome P450 (page 249) mRNA known as CYP2B1. The gene for this protein is activated in the liver by the barbiturate phenobarbital and hence a lot of CYP2B1 mRNA is made.
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