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gene construct isolate fertilized eggs isolate fertilized eggs

nucleus derived from the sperm inject "gene construct"

inject "gene construct"

nucleus derived from the sperm nucleus derived from the egg implant eggs into foster mother implant eggs into foster mother test for transgene pups

Bgl I

mouse metalothionein 5'-flanking sequence

Bgl I

mouse metalothionein 5'-flanking sequence

rat growth hormone structural gene

Bam HI

plasmid rat growth hormone structural gene

Bam HI

plasmid digest with Bgl I and Bam HI

BgI I

heavy metal ion enhancer sequence

Bam HI

inject eggs implantation pups fed Zn++

Figure 7.16. (a). Transgenic mouse carrying a foreign gene. (fa). the metalothionein gene contains a heavy-metal ion enhancer sequence. The + mice carry the transgene while littermates without the transgene are indicated by -.

exon exon exon

cross-over region —^ cross-over region genomic clone gene in chromosome genomic clone

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