Laboratory Detection Of Monocyteplatelet Aggregation

Monocytes are readily identifiable by whole-blood flow cytometry based on light-scatter properties and by using a monocyte-specific monoclonal antibody (e.g., the lipopoly-saccharide receptor CD14) (7,9,29-31). Leukocyte-platelet aggregates may be identified by the detection of platelet-specific markers (e.g., CD41, CD61, or CD42b) (29,31) on

Time from Symptom Onset

Fig. 2. Monocyte-platelet aggregates are an early marker ofMI. In patients presenting with MI, mono-cyte-platelet aggregates were highest within 4 h of onset. (Adapted with permission from ref. 9.)

Time from Symptom Onset

Fig. 2. Monocyte-platelet aggregates are an early marker ofMI. In patients presenting with MI, mono-cyte-platelet aggregates were highest within 4 h of onset. (Adapted with permission from ref. 9.)

monoyctes. Circulating monocyte-platelet aggregates may be expressed as a proportion of overall circulating monocytes or, using beads, absolute counts. Figure 3 demonstrates how monocyte-platelet aggregates are identified using flow cytometry.

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