The Lipoarabinomannan Glycolipid of Mycobacterium tuberculosis Progress in Total Synthesis via nPentenyl Orthoesters

Jayaprakash, and Siddhartha Ray Chaudhuri Natural Products and Glycotechnology Research Institute Inc. (NPG), 595F Weathersfield Road, Pittsboro, NC 27312 The lipoarabinomannan (LAM) cell surface glycolipid of Mycobacterium tuberculosis anchors a complex, highly branched glycan comprising arabinofuranan, mannan, and mannose-capped arabinan domains. This multifaceted architecture mirrors a multifaceted biological profile that implicates LAM in a wide range of...

Development of a Carbohydratebased Vaccine against Leishmaniasis

Lipophosphoglycans (LPGs) are the ubiquitous epitopes found on the surface of Leishmania parasites (Fig. 4) (57). The LPG contains three constituents a glycosylphosphatidylinositol (GPI) anchor, a repeating phosphorylated disaccharide, and a tetrasaccharide cap. Different leishmania species are distinguished by minor modifications in their backbone and by the number of repeating units. The phosphoglycan portion of the LPG has been shown to be a disease-promoting antigen (58-63) sparking much...

Factors affecting the efficacy of glycoconjugate vaccines

Identity of the individual glycan and carrier protein components 34 Glycan identity is best confirmed by NMR spectroscopy as the method is able to distinguish subtle structural changes and provide quantitative information on O-acetylation. Immunological approaches, combined with compositional data, can be used but immunological cross-reactivity can exist between serotypes. Immunochemical methods demonstrate identity of the carrier protein, but peptide mapping, mass spectrometry and size...