Regeneration Systems of UDPGal UDPGlc and UDPGlcA

UDP-Glc, the central sugar nucleotide in cells, can be prepared from UTP and Glc-1-P in the presence of glucose-1-phosphate uridylyltransferase (GalU, EC (66,67) or from sucrose and UDP with sucrose synthase (SusA, EC (Scheme 3) (59,68-70). UDP-Gal can be prepared from UDP-Glc by C4 epimeriza-tion using GalE (71), from galactose-1-phosphate (Gal-1-P) and UTP, or from UDP-Glc and Gal-1-P using GalPUT (54,72,73). Besides the pioneering work of Whitesides, Wong, and their collaborators, Wang et al. have also applied five recombinant enzymes to regenerate UDP-Gal (Scheme 4) (74,75). Finally, UDP-GlcA can be readily prepared by the nicotinamide adenine dinucleotide-dependent oxidation of the C6 hydroxyl group of UDP-Glc catalyzed by UDP-glucose 6-dehydrogenase (UGD, EC (76) (Scheme 5).

Was this article helpful?

0 0
Appetite Antidote

Appetite Antidote

Discover How You Can Free Yourself FromĀ  Uncontrolled Habits And Get Your Eating Under Control Once And For All! This Book Is One Of The Most Valuable Resources In The World When It Comes To Ways To Reclaime Your Rightful Body. Sound eating isn't about rigid nutrition doctrines, staying unrealistically skinny, or depriving yourself of the foods you adore.

Get My Free Ebook

Post a comment