Serum Sample Preparation

One of the advantages of CE is the simplicity of the sample preparation. For example, sometimes the sample can be introduced directly without preparation. However, due to matrix effects, analysis of drugs in serum often requires special preparation such as dilution, protein precipitation, or extraction. Drugs such as the antifungal fluconazole have been analyzed after a variety of sample preparation methods (7) to illustrate the differences in speed, linearity, detection limits, and precision....

Basic Principles Of Capillary Electrophoresis

Fundamentals of Electrophoresis Capillary electrophoresis (CE) is a special case of using an electrical field to separate the components of a mixture. Electrophoresis in a capillary is differentiated from other forms of electrophoresis in that it is carried out within the confines of a narrow tube. To understand the behavior of molecules under the influence of an electrical field inside a capillary it is essential to understand the phenomena that result from the geometry of a capillary. It...

Info

Correlation of cIEF and HPLC results. Hb A1c results were compared in two sets of samples analyzed by cIEF and ion exchange HPLC. Samples in both (A) and (B) were prepared from erythrocytes incubated in saline overnight prior to analysis by HPLC. Samples analyzed by cIEF were prepared from erythrocytes incubated in saline for 18 h (A) or 6 h (B) prior to analysis. Good correlation was observed in both comparisons, though both proportional (slope) and fixed (intercept) bias were affected...

Applications

Since CE-MS applications have already been presented and reviewed for analysis of large macromolecules like peptides (see Chapter 16), proteins, nucleotides, and oligosaccharides (18,20,23), the present application review will be restricted to small molecules like drugs, byproducts, and metabolites. This section will concentrate on four major modes 1. Capillary zone electrophoresis (CZE) 3. Micellar electrokinetic chromatography (MEKC) and The use of in-source collision induced dissociation is...

Review Of Coatings

It is well-established that covalently coated capillaries have a longer operational lifetime and are preferred to noncovalent methods for minimizing the effect of surface silanols. Typically, the coating protocol for covalently coated capillaries involves a step to activate the surface groups of the fused silica capillary. The goal of this activation is to make available a high density of surface silanols that are ionized. The activation step is followed by a silanization reaction that leaves...

References

Tiselius, A. (1930) The moving boundary method of studying the electrophoresis of proteins. Ph.D. thesis, Nova Acta Regiae Societatis Scientiarum Upsaliensis, Ser IV, Vol. 17, No. 4, Almqvist & Wiksell, Uppsala, Sweden, pp. 1-107. 2. Hjerten, S. (1967) Free zone electrophoresis. Chromatogr. Rev. 9, 122-219. 3. Jorgenson, J. W. and Lukacs, K. D. (1981) Zone electrophoresis in open tubular glass capillaries. Anal. Chem. 53, 1298-1302. 4. Jorgenson, J. W. and Lukacs, K. D. (1981)...

Introduction

The development of methods for the amplification and detection of specific regions of the DNA molecule using the polymerase chain reaction PCR has resulted in rapid and dramatic advances in biochemical analysis 1 . With the advent of the PCR it is now possible to easily produce analytically significant amounts of a specified DNA product. A typical PCR reaction can produce microgram quantities of target DNA, allowing rapid and efficient screening of genetic defects, cancer susceptibility, and...