Enrichment and Isolation Procedures

Media and culture conditions are the same as described for Chro-matium species. Prerequisite for the successful enrichment and isolation of Thiodictyon from anoxic water or mud samples is the microscopic detection of the bacterium in the particular sample. Since in its natural habitats Thiodictyon elegans usually grows in the form of readily recognizable netlike cell aggregates, recognition of this bacterium is relatively easy.

For the isolation of pure cultures, it is advisable to prepare both liquid enrichment cultures and agar shake dilution cultures that are directly inoculated from the sample of the natural habitat. In liquid enrichment cultures, Thiodictyon may be outgrown by other purple sulfur bacteria containing gas vesicles. The culture medium and incubation conditions are the same as those described for Lamprocystis species.

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