Enrichment and Isolation Procedures

Methods for enrichment and isolation are the same as those described for the genus Chromatium. Thiohalocapsa species often form dense purple layers in microbial mats of hypersaline environments with shallow waters at salinities from 10 to 20%, such as salterns, shallow salt lakes, and evaporitic lagoons. They are also found at the surface of sediments below top layers of salt deposits. Thiohalocapsa species can be enriched from samples of the natural environment using media composed of natural brines, as described by Caumette et al. (1991). They can be purified by repeated deep-agar dilution series. After achievement of pure cultures, a single colony is transferred into synthetic media containing 7-10% NaCl (Caumette et al., 1991).

0 0

Post a comment