For all three assays, analysis of covariance (ANCOVA) was used to detect genetic phenotypic variation due to food source and gender, with body weight and total proteins as covariates. The results of feeding flies for 10 d in the control, palmitic acid, soy, and beef diets are shown in Table 1. The seven dietary conditions used in this chapter and when the assays were performed are summarized in Fig. 1. The significant results (i.e., p < 0.05) of these metabolic experiments were that triglycerides were significantly lowered in female flies and pooled male and female flies after 10 d on the beef diet (p = 0.0233 and p = 0.0075, respectively). Also, the total protein significantly increased almost two-fold in male flies fed soy for 10 d (p = 0.0132) and significantly increased by more than 50% in pooled flies fed beef for 10 d (p = 0.001). None of the other conditions produced significant effects in triglyceride levels or total proteins, and none of the three diets significantly affected live weight. The latter result is significant because it shows that it is not simply the weight of the flies that is being affected.
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