Measuring the Time Between Egg Laying to Eclosion as Adults

In the adult longevity studies, every 2 d the adults were transferred to fresh food in a new cage and the number of dead males and females were counted. The old food with eggs was cut into pieces containing no more than 50 eggs and placed into empty 25 mL vials to determine the fecundity of the parents and to measure the amount of time it takes for the progeny to progress from egg laying to adult. Also, virgin males and females were collected from the vials at least twice a day and kept in separate vials with control food (no more than 20 male or female flies per vial) to determine the life span of these flies. We found that some dietary conditions during larval life affected the life span of the flies. However, these experiments are beyond the scope of this chapter and will be presented at a later time.

The time between egg laying to emergence was shortest for the control food (14.5 ±1.5 d) (Fig. 2A), and longest for the palmitic acid food (20.0 ± 2.8 d) (Fig. 2B). Soy and beef diets caused intermediate developmental delays (16.8 ± 3.5 and 17.4 ± 2.3 d, respectively) (Fig. 2C,D, respectively).

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