Oliver Medvedik and David A Sinclair

The diet known as caloric restriction (CR) has been known for 70 yr to extend the life span of rodents (1). CR can also extend life span in a broad range of other species as well, from unicellular organisms (2,3), to invertebrates (4) and most likely primates, as well (5). The budding yeast Saccharomyces cerevisiae is a useful model for the study of pathways that determine life span in response to dietary intake. Here, we describe how to calorically restrict yeast, the methods used to determine...

Preparation of Yeast for Replicative Life Span Analysis

2.2X supplemented yeast extract peptone YEP media 22.2 g L Bacto-yeast extract Becton, Dickinson and Company, Franklin, NJ , 44.4 g L Bacto-peptone Becton, Dickinson and Company, Franklin, NJ in water and filter sterilized with a 0.22-pm vacuum filter. Supplement the media by adding 40 mL L 0.5 adenine solution and 20 mL L for the remaining 1 amino acid solutions see Notes 1-3 . 2. 4 agar Bacto-agar Becton, Dickinson and Company, Franklin, NJ 8 g of agar is added into 200 mL of water and...